GSGF 904(Gelified Skin Growth Factor 904)General Data & References Veterinary Data1.00 GENERAL OBSERVATIONS1.01 tested in vitro on different cell types, the titrated extracts proved the following main properties :1.02 Rapid & healthy epithelialisation - increase in wound contraction rate GSGF 904 has remarkable properties which accelerate the epithelialisation process, increasing wound contraction rate, without skin proliferation or proud flesh. 1.03 pH balanced and absorption Due to its specific pH balanced isotonic saline base (deneutralised), GSGF 904 is rapidly and fully absorbed by the wound thus accelerating the epithelialisation process, whereas traditional greasy creams and ointments are more slowly and partially absorbed leaving unabsorbed residues on the surface. These ointment and cream residues oxidise with the body temperature, promoting irritation of epithelial cells, infection, and interfering with the healing process. 1.04 Protective barrier effect GSGF 904 has a protective barrier effect which guarantees that the wound has correct percentage of moisture needed for effective healing, reducing the risk of skin proliferation or proud flesh (e.g. epithelialisation process may stop or be altered if a wound is too dry. If a wound is too moist, there may be irritation and skin proliferation or proud flesh - as a result of excessive suppuration). 1.05 Insect repellent properties Several in vivo field tests have shown insect repellent properties while using GSGF 904. According to the literature, a blend of molecules contained in the formula are involved in this particular process. This insect repellent activity is however linked to skin absorption process (between 40 min. and 120 min). 1.06 Bactericidal The GSGF 904 synergistic bactericidal complex reduces the risk of bacterial attack without interfering with the different phases of the epithelialisation process, as GSGF 904 remains non-mutagenic (lack of cytogenotoxicity). 1.07 Non-Mutagenic and Hair re-growth Moreover, the hair grows back to its original colour, even when there has been extensive loss of cutaneous tissue or when the wounds are deep. This unusual feature could be explained by the fact that - when applied as recommended - GSGF 904 is devoid of mutagenicity (cytogenotoxiticy) and does not alter or denature the epithelial cells. So cutaneous injuries are covered by cells which are genetically and completely identical to the cells that were initially available. Tested on strains originating from the Laboratory of Professor B. AMES, Biochem. Dpt., California University, USA, it has been observed through this particular study (*) that - with or without metabolic activation when compared versus negative controls - there is no cytogenotoxic effect. (*) Study performed according OECD Guideline 471 « Genetic Toxicology - Reverse Mutation Assay », ANOVA and DUNNETT tests. 2.00 SPECIAL OBSERVATIONSWhen GSGF 904 was in vivo tested on animals (field tests), the healing process was accelerated considerably due to the following properties :2.01 «in vitro» Evaluation of the activity In a parallel direction, GSGF 904 has been subject to extensive research performed on an «in vitro» 3-dimensional skin model to evaluate : cell viability (MTT test), quantification of Tumor Necrosis Factor alpha (TNF alpha), Interleukin 1 alpha (IL-1alpha), Interleukin 10 (IL-10), Interleukin 8 (IL-8), Interleukin 12 (IL-12), Prostaglandin E2 (PGE2). Cfr. scientific ref. 6.00 2.02 Applications GSGF 904 must be applied to the wound as soon as possible in order to prevent infection and, above all, the destruction or alteration of the available surface cells. The quality of the available cells is very important as this hydrogel enhances the cell multiplication process (the fresher the wound, the faster the healing). GSGF 904 is also recommended for wounds which have been unsuccessfully treated with other conventional products. In some cases, it is advisable to scrape the proud flesh from the wound before applying GSGF 904 in order to obtain a base of good epithelial cells for an optimum result. WARNINGİ! Copyright 1992-1995-1997 - Maximilian Zenho & Co. - Brussels (Belgium). Reader is hereby notified that reproduction, duplication or diffusion of the information contained in the present confidential file is strictly prohibited. This confidential information is intended for internal use only and not for marketing or commercial purposes.  |